Novel Bio-functional Magnesium Coating on Porous Ti6Al4V Orthopaedic Implants: In vitro and In vivo Study

IMR OpenIR

	Novel Bio-functional Magnesium Coating on Porous Ti6Al4V Orthopaedic Implants: In vitro and In vivo Study
	Li, Xiaokang; Gao, Peng; Wan, Peng; Pei, Yifeng; Shi, Lei; Fan, Bo; Shen, Chao; Xiao, Xin; Yang, Ke; Guo, Zheng; Guo, Z (reprint author), Fourth Mil Med Univ, Xijing Hosp, Dept Orthopaed, Xian 710032, Peoples R China.; Yang, K (reprint author), Chinese Acad Sci, Inst Met Res, Shenyang 110016, Peoples R China.
	2017-01-19
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	7 页码:-
摘要	Titanium and its alloys with various porous structures are one of the most important metals used in orthopaedic implants due to favourable properties as replacement for hard tissues. However, surface modification is critical to improve the osteointegration of titanium and its alloys. In this study, a bioactive magnesium coating was successfully fabricated on porous Ti6Al4V by means of arc ion plating, which was proved with fine grain size and high film/substrate adhesion. The surface composition and morphology were characterized by X-ray diffraction and SEM equipped with energy dispersive spectroscopy. Furthermore, the in vitro study of cytotoxicity and proliferation of MC3T3-E1 cells showed that magnesium coated porous Ti6Al4V had suitable degradation and biocompatibility. Moreover, the in vivo studies including fluorescent labelling, micro-computed tomography analysis scan and Van-Gieson staining of histological sections indicated that magnesium coated porous Ti6Al4V could significantly promote bone regeneration in rabbit femoral condylar defects after implantation for 4 and 8 weeks, and has better osteogenesis and osteointegration than the bare porous Ti6Al4V. Therefore, it is expected that this bioactive magnesium coating on porous Ti6Al4V scaffolds with improved osteointegration and osteogenesis functions can be used for orthopedic applications.; Titanium and its alloys with various porous structures are one of the most important metals used in orthopaedic implants due to favourable properties as replacement for hard tissues. However, surface modification is critical to improve the osteointegration of titanium and its alloys. In this study, a bioactive magnesium coating was successfully fabricated on porous Ti6Al4V by means of arc ion plating, which was proved with fine grain size and high film/substrate adhesion. The surface composition and morphology were characterized by X-ray diffraction and SEM equipped with energy dispersive spectroscopy. Furthermore, the in vitro study of cytotoxicity and proliferation of MC3T3-E1 cells showed that magnesium coated porous Ti6Al4V had suitable degradation and biocompatibility. Moreover, the in vivo studies including fluorescent labelling, micro-computed tomography analysis scan and Van-Gieson staining of histological sections indicated that magnesium coated porous Ti6Al4V could significantly promote bone regeneration in rabbit femoral condylar defects after implantation for 4 and 8 weeks, and has better osteogenesis and osteointegration than the bare porous Ti6Al4V. Therefore, it is expected that this bioactive magnesium coating on porous Ti6Al4V scaffolds with improved osteointegration and osteogenesis functions can be used for orthopedic applications.
部门归属	[li, xiaokang ; gao, peng ; pei, yifeng ; shi, lei ; fan, bo ; shen, chao ; xiao, xin ; guo, zheng] fourth mil med univ, xijing hosp, dept orthopaed, xian 710032, peoples r china ; [wan, peng ; yang, ke] chinese acad sci, inst met res, shenyang 110016, peoples r china
学科领域	Multidisciplinary Sciences
资助者	National Natural Science Foundation of Youth Fund [51501223]; National High-tech R & D Program (863Program) [2015AA033702]; Shaanxi Science & Technology Research and Development Program [2015GY158, 2016GY198]
收录类别	SCI
语种	英语
WOS记录号	WOS:000392219700001
引用统计	被引频次：56[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/78335
专题	中国科学院金属研究所
通讯作者	Guo, Z (reprint author), Fourth Mil Med Univ, Xijing Hosp, Dept Orthopaed, Xian 710032, Peoples R China.; Yang, K (reprint author), Chinese Acad Sci, Inst Met Res, Shenyang 110016, Peoples R China.
推荐引用方式 GB/T 7714	Li, Xiaokang,Gao, Peng,Wan, Peng,et al. Novel Bio-functional Magnesium Coating on Porous Ti6Al4V Orthopaedic Implants: In vitro and In vivo Study[J]. SCIENTIFIC REPORTS,2017,7:-.
APA	Li, Xiaokang.,Gao, Peng.,Wan, Peng.,Pei, Yifeng.,Shi, Lei.,...&Yang, K .(2017).Novel Bio-functional Magnesium Coating on Porous Ti6Al4V Orthopaedic Implants: In vitro and In vivo Study.SCIENTIFIC REPORTS,7,-.
MLA	Li, Xiaokang,et al."Novel Bio-functional Magnesium Coating on Porous Ti6Al4V Orthopaedic Implants: In vitro and In vivo Study".SCIENTIFIC REPORTS 7(2017):-.