One-step fabrication of a self-driven point-of-care chip by femtosecond laser direct writing and its application in cancer cell H<sub>2</sub>O<sub>2</sub> detection via semiconductor-based SERS

doi:10.1016/j.talanta.2024.126483

IMR OpenIR

	One-step fabrication of a self-driven point-of-care chip by femtosecond laser direct writing and its application in cancer cell H₂O₂ detection via semiconductor-based SERS
	Wang, Yue 1; Lai, Bo 2; Yu, Zhi 3; Xu, Zhangrun 1
通讯作者	Xu, Zhangrun(xuzr@mil.neu.edu.cn)
	2024-10-01
发表期刊	TALANTA
ISSN	0039-9140
卷号	278 页码:10
摘要	Self-driven microfluidic systems have attracted significant attention and demonstrated great potential in the field of point-of-care (POC) testing due to their device simplicity, low power consumption, increased portability, and reduced sample consumption. To develop POC detection chips with diverse characteristics that meet different requirements, there is a strong demand for feasible strategies that enable easy operation and reduce processing time. Here, a one-step processing approach using femtosecond laser direct writing technology was proposed to fabricate a capillary-actuated POC microfluidic chip. The driving force of the chip is highly dependent on its surface wettability, which can be easily adjusted by changing the laser processing parameters. This POC microfluidic chip allowed for the detection of intracellular H2O2 through a catalytic reaction system that incorporated 5-aminosalicylic acid -sensitized colloidal TiO2 nanoparticles and horse radish peroxidase, with integrating semiconductor-based surface-enhanced Raman scattering (SERS) quantitative technique. The concentration of H2O2 was determined by the SERS signal of the catalytic products in the microfluidic chip, resulting in rapid detection with minimal sample consumption. Our method provides a simple, feasible, and alternative strategy for POC testing of H2O2, with a linear range of 10(-2)similar to 10(-6) M and a limit of detection of 0.55 mu M. This approach was successfully applied to rapid detection of intracellular H2O2 in MCF-7 breast cancer cells with high sensitivity and minimal sample consumption. Additionally, this study not only demonstrates the exceptional advantages of femtosecond laser processing technology in fabricating diverse microfluidic chips for various applications, but also presents an efficient POC testing strategy for detecting cell signaling molecules.
资助者	National Natural Science Foundation ; P. R. China ; Fundamental Research Funds for the Central Universities ; Liaoning Province science and technology plan project
DOI	10.1016/j.talanta.2024.126483
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation[22174012] ; National Natural Science Foundation[21874015] ; P. R. China ; Fundamental Research Funds for the Central Universities[N2205014] ; Liaoning Province science and technology plan project[2022 -MS -011]
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:001265915400001
出版者	ELSEVIER
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/188058
专题	中国科学院金属研究所
通讯作者	Xu, Zhangrun
作者单位	1.Northeastern Univ, Coll Sci, Dept Chem, Shenyang 110819, Peoples R China 2.Tongji Univ, Inst Precis Opt Engn IPOE, MOE Key Lab Adv Microstruct Mat, Shanghai 200092, Peoples R China 3.Chinese Acad Sci, Inst Met Res, Shenyang Natl Lab Mat Sci, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yue,Lai, Bo,Yu, Zhi,et al. One-step fabrication of a self-driven point-of-care chip by femtosecond laser direct writing and its application in cancer cell H₂O₂ detection via semiconductor-based SERS[J]. TALANTA,2024,278:10.
APA	Wang, Yue,Lai, Bo,Yu, Zhi,&Xu, Zhangrun.(2024).One-step fabrication of a self-driven point-of-care chip by femtosecond laser direct writing and its application in cancer cell H₂O₂ detection via semiconductor-based SERS.TALANTA,278,10.
MLA	Wang, Yue,et al."One-step fabrication of a self-driven point-of-care chip by femtosecond laser direct writing and its application in cancer cell H₂O₂ detection via semiconductor-based SERS".TALANTA 278(2024):10.