In vitro and in vivo studies on bacteria and encrustation resistance of heparin/poly-L-lysine-Cu nanoparticles coating mediated by PDA for ureteral stent application

doi:10.1093/rb/rbac047

IMR OpenIR

	In vitro and in vivo studies on bacteria and encrustation resistance of heparin/poly-L-lysine-Cu nanoparticles coating mediated by PDA for ureteral stent application
	Awonusi, Bukola O.1,2; Li, Jianzhong 3; Li, Hongwei 3; Wang, Zhenyu 3; Yang, Ke 1; Zhao, Jing 1
通讯作者	Yang, Ke(kyang@imr.ac.cn) ; Zhao, Jing(zhaojing@imr.ac.cn)
	2022-04-08
发表期刊	REGENERATIVE BIOMATERIALS
ISSN	2056-3418
卷号	9 页码:11
摘要	Ureteral stents are commonly utilized as a medical device to aid the flow of urine. However, biofilm formation and encrustation complications have been clinical problems. To overcome this challenge, heparin/poly-L-lysine-copper (Hep/PLL-Cu) nanoparticle was immobilized on a dopamine-coated polyurethane surface (PU/NPs). The stability and structural properties of the nanoparticles were characterized by Zeta potential, poly dispersion index, transmission electron microscopy, atom force microscopy and contact angle. The surface composition, antibacterial potency, encrustation resistance rate and biocompatibility of PU/NPs were investigated by scanning electron microscope, X-ray photoelectron spectroscopy, antibacterial assay and MTS assay, respectively. In addition, the anti-encrustation property was studied by implanting coated NPs stents in the rat bladder for 7 days. It was shown that the size and distribution of Hep/PLL-Cu nanoparticles were uniform. PU/NPs could inhibit Proteus mirabilis proliferation and biofilm formation, and exhibit no cytotoxicity. Less encrustation (Ca and Mg salt) was deposited both in vitro and in vivo on samples, demonstrating that the NPs coating could be a potential surface modification method of ureteral material for clinical use.
关键词	ureteral stents nanoparticles biofilm encrustation
资助者	Liaoning Science and Technology Program
DOI	10.1093/rb/rbac047
收录类别	SCI
语种	英语
资助项目	Liaoning Science and Technology Program[2020JH2/10300159]
WOS研究方向	Materials Science
WOS类目	Materials Science, Biomaterials
WOS记录号	WOS:000835421500004
出版者	OXFORD UNIV PRESS
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/174624
专题	中国科学院金属研究所
通讯作者	Yang, Ke; Zhao, Jing
作者单位	1.Chinese Acad Sci, Shi Changxu Innovat Ctr Adv Mat, Inst Met Res, Shenyang 110016, Peoples R China 2.Univ Sci & Technol China, Sch Mat Sci & Engn, Hefei 230026, Peoples R China 3.Gen Hosp Northern Theater Command, Dept Urol, Shenyang 110840, Peoples R China
推荐引用方式 GB/T 7714	Awonusi, Bukola O.,Li, Jianzhong,Li, Hongwei,et al. In vitro and in vivo studies on bacteria and encrustation resistance of heparin/poly-L-lysine-Cu nanoparticles coating mediated by PDA for ureteral stent application[J]. REGENERATIVE BIOMATERIALS,2022,9:11.
APA	Awonusi, Bukola O.,Li, Jianzhong,Li, Hongwei,Wang, Zhenyu,Yang, Ke,&Zhao, Jing.(2022).In vitro and in vivo studies on bacteria and encrustation resistance of heparin/poly-L-lysine-Cu nanoparticles coating mediated by PDA for ureteral stent application.REGENERATIVE BIOMATERIALS,9,11.
MLA	Awonusi, Bukola O.,et al."In vitro and in vivo studies on bacteria and encrustation resistance of heparin/poly-L-lysine-Cu nanoparticles coating mediated by PDA for ureteral stent application".REGENERATIVE BIOMATERIALS 9(2022):11.