KAI1 reverses the epithelial-mesenchymal transition in human pancreatic cancer cells

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	KAI1 reverses the epithelial-mesenchymal transition in human pancreatic cancer cells
其他题名	KAI1 reverses the epithelial-mesenchymal transition in human pancreatic cancer cells
	Liu Xu; Guo XiaoZhong; Li HongYu; Chen Jiang
	2019
发表期刊	HEPATOBILIARY & PANCREATIC DISEASES INTERNATIONAL
ISSN	1499-3872
卷号	18 期号:5 页码:471-477
摘要	Background: Epithelial-mesenchymal transition (EMT) plays an important role in pancreatic cancer (PC). In the present study, we investigated the effects of KAI1 gene overexpression on the EMT of human PC cell lines, MIA PaCa-2 and PACN-1.
其他摘要	Background: Epithelial-mesenchymal transition (EMT) plays an important role in pancreatic cancer (PC). In the present study, we investigated the effects of KAI1 gene overexpression on the EMT of human PC cell lines, MIA PaCa-2 and PACN-1. Methods: Plasmids overexpressing KAI1 and pCMV were transfected into MIA PaCa-2 and PACN-1 cells, respectively. After selection of differently transfected cells by G418, KAI1 protein levels were examined by Western blotting, and transfected cells were renamed as MIA PaCa-2-K, MIA PaCa-2-p, PACN-1-K and PACN-1-p. Wound healing and Transwell migration assays were then performed comparing the two groups of cells. EMT-related markers were analyzed by Western blotting. Results: The percentage of wound closure significantly decreased in MIA PaCa-2-K cells compared with MIA PaCa-2-p and MIA PaCa-2 cells after 24, 48 and 72 h (P < 0.05). In PACN-1-K cells, the percentage of wound closure significantly decreased as well (P < 0.05). Numbers of invading MIA PaCa-2, MIA PaCa-2-p and MIA PaCa-2-K cells were determined as 48.0 ±15.4, 50.0 ±12.4, and 12.0 ±3.8, respectively. The corresponding numbers of invading PACN-1, PACN-1-p and PACN-1-K cells were 29.0 ±10.6, 31.0 ±11.4, and 8.0 ±4.2, respectively. KAI1 overexpression induced a significant upregulation of E-cadherin and also significant downregulation of Snail, vimentin, matrix metalloproteinase 2 (MMP2) and MMP9 (all P < 0.05) in PC cells. Conclusions: KAI1 reversed EMT-related marker expression and inhibited migration and invasion of PC cells. Thus, KAI1 might represent a novel potential therapeutic target for PC.
关键词	EXPRESSION METASTASIS ACTIVATION VIMENTIN ADHESION SNAIL KAI1 Epithelial-mesenchymal transition Pancreatic cancer
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of China] ; [Science Foundation of Liaoning]
CSCD记录号	CSCD:6604826
引用统计	被引频次：1[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/157688
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Liu Xu,Guo XiaoZhong,Li HongYu,et al. KAI1 reverses the epithelial-mesenchymal transition in human pancreatic cancer cells[J]. HEPATOBILIARY & PANCREATIC DISEASES INTERNATIONAL,2019,18(5):471-477.
APA	Liu Xu,Guo XiaoZhong,Li HongYu,&Chen Jiang.(2019).KAI1 reverses the epithelial-mesenchymal transition in human pancreatic cancer cells.HEPATOBILIARY & PANCREATIC DISEASES INTERNATIONAL,18(5),471-477.
MLA	Liu Xu,et al."KAI1 reverses the epithelial-mesenchymal transition in human pancreatic cancer cells".HEPATOBILIARY & PANCREATIC DISEASES INTERNATIONAL 18.5(2019):471-477.